ABSTRACT
Objective:To investigate the inhibiting effect of Sufuning Lotion (SFN) on bladder carcinoma T24 cells.Methods:Trypan blue exclusion test was performed to observe the killing effect of 2 mg/ml SFN at different time points (20, 40, 60, 80, 100 min) on human bladder carcinoma T24 cells; the inhibiting effect of SFN with different concentrations (8.0, 12.0, 18.0, 27.0, 40.5 μg/ml) for 48 h on proliferation of T24 cells was assessed by using methyl thiazolyl tetrazolium (MTT) assay. The half inhibitory concentration ( IC50) was identified. T24 cells were treated with IC50 SFN for 24, 48, 72 h, and then the change of proliferation inhibition rate of T24 cells was detected. The nude mice subcutaneous model (30 mice) and intraperitoneal tumor xenograft model (30 mice) were prepared according to T24 cells inoculated method. After inoculation for 24 h, both animal models were divided into 5 groups with 6 animals in each group based on the random number method, including the control group (0.9% NaCl solution), the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group, and then the control group and three SFN groups were intraperitoneally injected for 6 d, while the mitomycin 1 mg/kg group was injected with 1 mg/kg mitomycin every 5 d for once, 2 times in total. The transplantable tumor volume of subcutaneous tumor xenograft model was measured per week and the mice were sacrificed after 4 weeks. Tumor tissues were taken out to measure the tumor weight and tumor growth inhibition ratio was also evaluated. The survival time of nude mice in intraperitoneal tumor xenograft model was recorded so as to calculate the life extension rate. Results:Trypan blue exclusion test showed that after the function of 2 mg/ml SFN for 20, 40, 60, 80, 100 min, the cell death rate was (17.83±1.56)%, (48.95±1.34)%, (67.46±1.44)%, (75.48±2.12)%, (89.41±1.35)%, respectively, and the difference was statistically significant ( F = 1 213.264, P < 0.01). MTT assay showed that SFN inhibited the proliferation of T24 cells in a concentration-dependent and time-dependent manner, and the IC50 of cell proliferation at 48 h was (14.36±0.35) μg/ml. After the function of 14.36 μg/ml SFN for 24, 48, 72 h, the proliferation inhibitory rate of T24 cells was (39.5±0.9)%, (50.6±0.7)%, (71.5±1.0)%, respectively, and differences was statistically significant ( F = 1 044.206, P < 0.01). After the nude mice was inoculated with T24 cells for 4 weeks, the tumor volume and tumor weight in the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group were lower than those in the control group [the tumor volume: (0.925±0.136) cm 3, (0.833±0.171) cm 3, (0.652±0.117) cm 3, (0.482± 0.120) cm 3 vs. (1.231±0.210) cm 3, respectively; the tumor weight: (1.56±0.20) g, (1.42±0.21) g, (1.19±0.22) g, (0.97±0.16) g vs. (1.98±0.30) g], and differences were statistically significant ( F = 20.153, P < 0.01; F = 17.325, P < 0.01); there were no significant differences in the tumor volume and weight between the SFN 400 mg/kg group and the mitomycin group ( t = 1.898, P = 0.069; t = 1.739, P = 0.094), the inhibition rate of subcutaneous tumor xenograft model was 20.94%, 28.28%, 39.66%, 51.14%, respectively in the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group. The survival time of intraperitoneal nude mice in the SFN groups and the mitomycin group was prolonged compared with that in the control group [(32.7±3.2) d, (34.0±4.5) d, (34.3±2.3) d, (35.3±2.0) d vs. (21.7±4.8) d], and there was a statistically significant difference ( F = 15.179, P < 0.01), the life extension ratio was 50.76%, 56.90%, 58.42%, 63.04%, respectively. Conclusion:SFN can inhibit the proliferation of T24 cells, and it has an anti-tumor effect on the T24-bearing nude mice.
ABSTRACT
Objective To investigate the efficacy and safety of intravesical instillation with Sufuning (SFN) lotion for prevention of postoperative recurrence of bladder cancer. Methods A total of 240 bladder cancer patients who were diagnosed as bladder cancer and accepted trans-urethral resection of bladder tumor from January 2010 to June 2016 in Shanxi Provincial Cancer Hospital were randomly divided into the experimental group (120 cases) and the control group (120 cases) according to the envelope method. The patients in the experimental group were treated with SFN lotion for immediate intravesical instillation(250 mg for once), and the patients in the control group were treated with pirarubicin (THP) for immediate intravesical instillation (30 mg for once). The patients of two groups were treated with intravesical chemotherapy once a week for 8 times, and the chemotherapy was performed once a month for 1 year. The recurrence rate, progression-free survival (PFS) rate, overall survival (OS) rate and recent side effects were compared between the two groups. Results The patients were followed up for 6 to 60 months. The median follow-up time was 36.5 months.In the experimental group,6 patients were lost and 8 patients were lost in the control group.The experimental group, the total recurrence rate was 26.3 % (30/114). The control group, the overall recurrence rate was 25.0 % (28/112) (χ2= 0.142, P = 0.781). Five years of PFS rate in the experimental group and the control group was 73.7 % (84/114) and 75.0 % (84/112) respectively, and there was no significant difference between the two groups (χ2= 2.011, P= 0.615). Five years of OS rate in the experimental group and the control group was 95.6 % and 92.9 % respectively, and there was no significant difference between the two groups (χ 2= 1.611, P= 0.425). The major side effects included chemical cystitis and hematuria. The incidence of chemical cystitis and hematuria in the experimental group was significantly lower than that in the control group(χ2=5.991,P=0.018;χ2=4.925,P=0.036).There was a statistically significant difference of the hematological side effects (blood routine changes) between the two groups (χ 2= 4.891, P= 0.032). Conclusion It is safe and effective for intravesical instillation of SFN lotion to prevent the recurrence of bladder cancer.